Proteomics Unit

...where Neurobiology meets Biotechnology

 

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Mass spectrometry is a powerful analytical technique that is used to identify and quantify compounds, and to elucidate the structure and chemical properties of molecules.

The Unit is specialized in

-identification and quantification of proteins from simple and complex samples,

-identification and quantification of post-translational modifications,

-identification and quantification of drugs and metabolites.

We also aim to identify biomarkers through proteomics and metabolomics techniques with the purpose of developing new prognosis and diagnosis methods, in collaboration with other R&D units at CNC and Biocant and external partners through our excellence networks.

This unit is equipped with state of the art technology for:

-uni- and bi-dimensional separation of drugs, metabolites, peptides and digested proteins from complex samples by capillary- to analytical-HPLC;

-mass spectrometry identification and quantification using distinct sample ionization and scan methods, in an hybrid instrument that takes advantage of the selectivity provided by the triple quadrupole and the sensitivity of the ion-trap.

4000 QTRAP mass spectrometer was acquired with founds from "Programa Nacional de Reequipamento Cientifico"

and the Proteomics Unit is member of portuguese national mass spectrometry network

Request information

Service Request Form

All samples submitted to the Unit will be processed in accordance with the Facility Policy and Users fees

 

Reference Price List(1)

(1)Reference Price List created according to general rules for equipment use and applied to "Services". Collaborators (including: CNC, Biocant, RNEM users and others) are encouraged to contact the unit for "Collaboration Price List"

 

Analysis

Price (€)

Sample pre-treatment    
  Filtration or zip-tip 10
  Filtration or zip-tip (>8 samples) 8
     
ESI    
  Small molecule 40
  Protein 75
     
nanoESI    
  Small molecule 60
  Protein 85
     
Fragmentation    
  per ion 10
     
Digestion    
  Spot/band (includes destain) 50
  Spot/band (includes destain) (>8 samples) 30
  Liquid samples (2)
     
LC-MSMS    
  LC-MSMS 150
  LC-MSMS (>8 samples) 100
  2D-LC-MSMS 200€+100€ x n(3)
     
iTRAQ    
  2-plex price available soon
  4-plex price available soon
  8-plex price available soon
     
Report    
  ProteinPilot Report 50
  PEAKS report 50
  Comprehensive analysis 100
     
Statistics    

 

Multivariate analysis

30€/h

Minimum of 2h

     
Metabolite quantification    
  1-7 samples* 100(4)
  8-20 samples* 75(4)
  >20 samples* inquire
  * plus method development, if applicable  
 

 

(2) Depends on protein content (>100€/100ug of protein content)

(3) n=number of fractions to run on RP-LC-MS/MS

(4) Does not include calibration curves

 

Equipment occupancy time-table (prevision)

 
April

 

 

 

 

Friday 1

 

 

 

 

ID59

Monday 4

Tuesday 5

Wednesday 6

Thursday 7

Friday 8

T

T

T

ID59

ID59

Monday 11

Tuesday 12

Wednesday 13

Thursday 14

Friday 15

T

T

T

ID59

ID59

Monday 18

Tuesday 19

Wednesday 20

Thursday 21

Friday 22

ID60

ID60

ID60

ID60

ID60

Monday 25

Tuesday 26

Wednesday 27

Thursday 28

Friday 29

ID61

ID61

ID61

ID61

ID61

M – Maintenance

T – Training

 
© 2008-2010 Proteomics Unit - Center for Neuroscience and Cell Biology